Join Natalia Markova, Principal Scientist, Malvern Panalytical for the Alderley Park Science Seminar Series.

This seminar will be delivered via Zoom. Link to be sent closer to the time.

Dynamic interactions involving biomolecules drive and regulate all biological processes, making interaction analysis a key area of academic and industrial research and development. A variety of biophysical techniques are used in this field, including Nuclear Magnetic Resonance (NMR), Isothermal Titration Calorimetry (ITC), biosensors (such as SPR and BLI) and fluorescence-based assays.

Over the years, clear trends in interaction and structural analyses have driven towards increased ease-of-use of advanced techniques, despite the increasing complexity of biomolecules and binding modes being studied.

While methodologies and technologies in interaction analysis continue to evolve, one fundamental prerequisite to the success remains constant: good control over the quality of samples and experimental conditions. Overlooking these requirements could result in poor performance of a biophysical technique, misleading and irreproducible results (such as poor SAR), and lack of convergence with orthogonal and complementary data generated during a project.

Case study examples will be given to illustrate how early profiling of the stability and homogeneity of samples, along with data-driven optimization of assay conditions impact on the quality of interaction analysis and progress of drug discovery projects.